EVALUATION OF EXTRACTS OF SOME NOXIOUS PLANTS AGAINST COFFEE BERRY DISEASE (Colletotrichum kahawae L.)

Serawit Handiso Melkato, Tesfaye Alemu

Abstract


Aqueous, alcoholic and dry extracts of five different noxious plants, namely, Senna occidentalis, Melia azendrachta, Parthenium hysterophorus, Calotropis procera and Argemone mexicana were tested in in vitro condition with the purpose of evaluating their inhibiting effects against C. kahawae. Data were collected on radial growth and percent inhibition. ANOVA had indicated that the aqueous and aqueous extracts of Melia azendrachta gave highest zones of inhibition followed by Senna occidentalis with 76.02%, 59.95%, respectively. Alcoholic extracts Calotropis procera and Senna occidentalis gave the highest zones of inhibition with 72.75% and 63.62%, respectively. Senna occidentalis had superior inhibition effect in both aqueous and ethanol extracts when applied 24 hours after inoculation with 83.37% and 80.67%. However, the aqueous extracts of all the botanicals tested were superior to Clotropis procera when applied 12 hours before inoculation of the fungus. Except ethanol extract of Senna occidentalis, the other botanicals were not statistically different from the control. This indicated that there is relationship between the time of application and efficacy of the antifungal compounds from these botanicals. However, the aqueous extracts of the seeds of these plants gave a better inhibitory effect than the leaf parts. Dry extracts of Senna occidentalis followed by Argemone mexicana gave better inhibitory effect against C. kahawae with 76.37% and 70.55%, respectively. Moreover, the extracts of Calotropis procera and Melia azendrachta had been with a promisingly higher inhibitory effect in this study. Therefore, in vitro evaluation and testing of these botanical plant extracts have antifungal compounds which reduced the mycelia growth of Colletotrichum kahawae.

Hence, further pot and field researches are deemed necessary on these botanicals for the effective control of C. kahawae.


Keywords


Aqueous extracts; Colletotrichum kahawa; Ethanol Extracts; inhibitory effect; noxious plants extracts.

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References


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